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Objective:To explored the mechanism of circ_0009910 in gastric cancer cells by detecting and analyzing the expression of it.Methods:qRT-PCR assay was used to detect the expression level of circ_0009910 in gastric cancer cell lines BGC823, SGC7901, AGS, MGC803 and MKN45. In circumstance, circ_0009910 siRNA was used to knock down circ_0009910 in BGC823 and AGS cells. The transfection efficiency was verified. In BGC823 and AGS cells knocked down by circ_0009910, cell proliferation was detected by MTT assay, colony formation was detected by plate assay, migration and invasion were detected by Transwell, and epithelial-mesenchymal transition (EMT) was detected by western-blotting. The software of SPPSS 18.0 was used to conduct statistical analysis.Results:The relative expression levels of circ_0009910 in BGC823, SGC7901, AGS, MGC803, and MKN45 were (7.238±0.895), (5.023±0.786), (4.184±0.356), (8.561±1.026), (3.478±0.301), which were significantly higher than normal gastric cancer ( P<0.01). After knocking down circ_0009910 by siRNA transfection, the BGC823, AGS cell viability, colony formation number, migration and invasion ability were significantly reduced compared with the control group ( P<0.01), the relative expressions of N-cadherin and Snail proteins were reduced, and E -Cadherin expression increased. Conclusion:Circ_0009910 is highly expressed in gastric cancer cells, and can promote gastric cancer cell proliferation, colony formation, migration, invasion and EMT.
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Objective To study the expression and significance of circ_0009910 in gastric cancer,and evaluate its correlation with clinicopathological characteristics and prognostic value.Methods From Feb.2010 to Nov.2014,there were 129 gastric cancer patients in Beijing Haidian Hospital.One hundred and twenty-nine cases of tumor tissues and 129 paracancerous non-tumor tissues were collected from gastric cancer patients,intraoperatively.The expression of circ_0009910 was detected by qRT-PCR experiment.Then the correlations of circ_0009910 with major clinicopathological characteristics were analyzed.The Kaplan-Meier method was used to analyze overall survival of patients.A Cox proportional hazards regression analysis was used for multivariate analyses of prognostic values.Results The expression level of circ_0009910 in tumor tissues of patients was significantly higher than that in paracancerous non-tumor tissues (P ＜0.01).The expression level of circ_0009910 in patients was significantly associated with clinical stage (P =0.032),distant metastasis (P =0.018) and differentiation (P =0.007),respectively.There was no obvious correlation of the expression level of circ_0009910 with age,gender,Lauren's classification,tumor diameter (P ＞0.05).And the expression level of circ_0009910 was higher in patients with advanced cancer (stage Ⅲ/Ⅳ),distant metastasis and poor differentiation.Survival rate of patients in positive group was significantly lower than negative group (P =0.001 5),with 5-year survival rate were about 29.5％ and 53.2％,respectively.circ_0009910 (P =0.006),distant metastasis (P =0.009) and differentiation (P =0.035) were independent risk factors of overall survival of patients,respectively.Conclusions The expression level of circ_0009910 in tumor tissues of gastric cancer patients was significantly higher than that in paracancerous non-tumor tissues,which was significantly correlated with clinical stage,distant metastasis and differentiation.Survival rate was lower in patients with higher expression level of circ_0009910.circ_0009910 was an independent risk factors of overall survival of patients.
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Objective To analyze the clinical efficacy and relative influence factors of self-expanding metallic stents (SEMS) in treating colorectal cancer obstruction.Methods Information of 47 patients received SEMS to treat colorectal cancer obstruction from Mar.2012 to Dec.2017 in Beijing Haidian Hospital were collected,who were then followed up in outpatient or by telephone.Effective rate,survival rate and complications were recorded and calculated by the software of SPSS 17.0.Chi-square test was used to analyze relative influence factors.Results Results shown that the clinical efficacy was 100％ (47/47).Patients' 30-day survival rate and 6-month survival rate were 87.2％ (41/47) and 68.1％ (32/47),respectively.After surgery,2 patients presented with perforation,3 patients presented with migration and 5 patients presented with reobstruction.Clinical stage of tumor and stent length were related with complications and survival.Therein,complications presented more in patients with advanced cancer.And the longer stent length,the higher mortality.Conclusions Clinical stage of tumor and stent length maybe risk factors of complications and survival of patients after SEMS surgery of colorectal cancer obstruction.
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Objective To explore the value and criteria for primary tumor resection in patients with stage Ⅳ rectal cancer. Methods The clinical data were retrospectively analyzed for 53 patients with stage Ⅳ rectal cancer undergoing primary tumor resection. Results All patients were graded according to Eastern Cooperative Oncology Group (ECOG) performance as status 1. Seventeen of 53 patients (32%) received palliative resection. Thirty-six patients received adjuvant radiotherapy and/or chemotherapy. The median follow-up was 14 months. Forty-seven patients had complete resolution of their symptoms related to the primary rectal cancer for a year. The median survival was 16 months and 2-year overall survival of 24.5%. On multivariate analysis, chemotherapy was a determinant of prolonged survival (P=0.046). Conclusions Resection of primary tumor in patients with stage Ⅳ rectal cancer can improve the quality of life. Patients who have a good performance status, minimal metastatic disease and normal preoperative liver function tests should undergo resective surgery and aggresive systemic chemotherapy.